The estrogen-dependent transcription of an endometrial proline-rich acidic protein PRAP and its ability to enhance endometrial estrogen responsiveness
Date Issued
2011
Date
2011
Author(s)
Fu Hsieh, Tsai-
Abstract
Mice were used as experimental animals to study the functional role of uterine PRAP and how its expression is regulated by ovarian steroids. Soluble proteins in the uterine luminal fluid (ULF) of estrous females were resolved by 2-D gel electrophoresis. The protein spot corresponding to PRAP on the gel slab was identified by proteomic analysis. However, PRAP was absent in the ULF of DES- treated immature mice. Among the sexual glands of adults, both PRAP and its transcript were exclusively expressed in the endometrial epithelium of estrous females. Uterine Prap expression changed during the estrous cycle, in which the transcript levels progressively increased from proestrus via estrus to early metestrus, after which they rapidly declined via late metestrus to a very low level in diestrus. Daily injection of estradiol (E2) at a dose of 0.1 μg•g-1•day-1 to immature females for 3 consecutive days markedly stimulated uterine Prap transcription, whereas transcription was not initiated in the animals that received an equal dose of diethylstilbestrol dipropionate (DES). Relative to the normal estrus female, prepubertal DES exposure decreases remarkedly in the uterine Prap expression when the animals became sexually matured. The E2-stimulated Prap transcription involved the α-type estrogen receptor; at higher E2 doses, a lower level of uterine Prap mRNA and a lower amount of PRAP protein in ULF were detected. Using the Ishikawa cell line as a surrogate model of endometrial epithelium, we demonstrated that exogenous PRAP in the milieu rapidly internalizes into the cells to enhance endometrial estrogen responsiveness.
Subjects
uterine luminal fluid
diethylstilbestrol
α-type estrogen receptor
estrogen responsiveness
Ishikawa cell line
Type
thesis
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