Bioactivity analysis of partial fragments within GMI, an immunomodulatory protein from Ganoderma microsporum
Date Issued
2014
Date
2014
Author(s)
Hsieh, Wan-Ling
Abstract
GMI, a fungal immunomodulatory protein (FIP) isolated from Ganoderma microsporum had been reported that it could interact with cell surface receptors and trigger downstream signal transduction. According to these evidences, FIPs exhibit potent immunomodulation and anti-tumor effect. In this study, we investigated the partial fragments within GMI and analyzed their bioactivities. By analyzing the secondary structure alignment and bioinformatics data, selected one α-helix and three β-sheet amino acid sequences. In order to get high quality and output target peptides, these peptides were produced by chemical synthesis. In terms of cell immune assay, wild type protein GMI and mutative protein GMI-L6C could enhance Jurkat T cells to secret IL-2 but α-helix-peptide and three β-sheet-peptides could not. By analyzing the results of anti-tumor tests, GMI and GMI-L6C could inhibit A549 viability and migration. Interestingly, α-helix-peptide could affect A549 viability and migration. However, three β-sheet-peptides could not inhibit A549 neither viability nor migration. Based on cell immune and anti-tumor assays, GMI-L6C exhibited better immunomodulatory and anti-tumor ability. It’s indicated dimerization is important to its bioactivity. By α-helix-peptide exhibiting anti-tumor activity, it suggested the importance of α-helix in anti-tumor activity of GMI. On the contrary, β-sheet-peptides didn’t have immunomodulatory and anti-tumor activity.
Subjects
真菌免疫調節蛋白質
GMI
胜肽
α-helix
β-sheet
細胞免疫
抗腫瘤
Type
thesis
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