Study in Cryopreservation of Six Marine Microalgae for Aquaculture by two-step protocol
Date Issued
2011
Date
2011
Author(s)
Chang, Yung-Cheng
Abstract
Cryopreservation at ultra-low (-196℃ in liquid nitrogen) has many advantages over the conventional maintenance of algae strains using serial subculture, it is becoming the method of choice for the long-term conservation of algae nowadays. This study determining the optimal protocol for cryopreservation of six marine microalgae : Nannochloropsis oculata, Tetraselmis sp., Seminavis gracilenta, Amphora exigua, Skeletonema costatum and Isochrysis galbana using the two-step method protocol with five cryoprotectant and five consentration are discussed. The results showed the best cryoprotectant for algae is species-specific. The highest post-thawed viability for N. oculata was 89% with 20% ethylene glycol(EG), for T. elliptica was 57% with 20% propylene glycol(PG), for S. gracilenta was 94% with 20% dimethyl sulfoxide(DMSO), for A. exigua was 90% with 20% dimethyl sulfoxide(DMSO) and for I. galbana was 72% with 15% glycerol(GLY). Two-step method protocol was not suitable for cryo-storage of Skeletonema costatum. The results showed that the nutritional composition of the microalgae were not significantly affected after cryopreservation.
Subjects
cryopreservation
cryoprotectant
marine microalgae
nutritional composition
SDGs
Type
thesis
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