WWP1 gene is a potential molecular target of human oral cancer
Journal
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Journal Volume
116
Journal Issue
2
Pages
221-231
Date Issued
2013
Author(s)
Abstract
Objective: This study investigates the oncogenic role of WWP1, an ubiquitin ligase linked to tumor promotion, in oral cancer. Study design: An array-based comparative genomic hybridization was used to detect chromosomal changes in 10 oral cancer specimens. An additional 59 specimens and 6 cultured oral cancer cells were further examined to evaluate changes in the DNA copy number and messenger RNA (mRNA) and protein expression of WWP1. Results: The copy number of the WWP1 gene and its mRNA levels were significantly increased in the oral cancer specimens. An elevated WWP1 gene expression was observed in 6 cultured oral cancer cell lines. Knockdown of the endogenous WWP1 using small hairpin RNA further showed that deficiency of WWP1 suppressed cell growth and caused apoptosis in oral cancer cells. Conclusion: Our results reveal that WWP1 might play an oncogenic role in oral cancer cells. ? 2013 Elsevier Inc. All rights reserved.
SDGs
Other Subjects
messenger RNA; small interfering RNA; ubiquitin protein ligase; WWP1 protein, human; adult; aged; apoptosis; article; cancer staging; carcinogenesis; cell proliferation; comparative genomic hybridization; copy number variation; exon; gene amplification; gene expression regulation; gene silencing; gene targeting; genetics; human; male; middle aged; mouth tumor; mutation; oncogene; pathology; squamous cell carcinoma; tumor cell line; Adult; Aged; Apoptosis; Carcinogenesis; Carcinoma, Squamous Cell; Cell Line, Tumor; Cell Proliferation; Comparative Genomic Hybridization; DNA Copy Number Variations; Exons; Gene Amplification; Gene Expression Regulation, Neoplastic; Gene Knockdown Techniques; Gene Targeting; Humans; Male; Middle Aged; Mouth Neoplasms; Mutation; Neoplasm Staging; Oncogenes; RNA, Messenger; RNA, Small Interfering; Ubiquitin-Protein Ligases
Type
journal article