In vitro study of SDF-1α-loaded injectable and thermally responsive hydrogels for adipose stem cell therapy by SDF-1/CXCR4 axis
Journal
Journal of Materials Chemistry B
Journal Volume
8
Journal Issue
45
Pages
10360-10372
Date Issued
2020
Author(s)
Abstract
Stem cell-based approaches have become a promising therapeutic strategy for treating ischemic diseases. The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis. Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA) were designed and fabricated to achieve local and sustained release of SDF-1α for ASC recruitment. Herein, the material structures, physical properties, gelation temperature, and gelation time of hydrogels with different compositions were determined. The incorporation of 0.9% HA in CS-based hydrogels not only enhanced the gelation time but also increased the strength of the hydrogels. In addition, the results revealed that the thermoresponsive and injectable CS/βGP/HA hydrogels showed good biocompatibility. In addition, the in vitro release profiles showed that the hydrogels achieved sustained release of SDF-1α over 7 days and enhanced ASC migration. The results revealed that the hydrogels with HA enhanced the sustained release effect compared with the hydrogel without HA, indicating that the HA content regulated the physical and release properties of the injectable hydrogels. Therefore, thermoresponsive and injectable CS/βGP/HA hydrogels may provide an alternative for treating ischemic diseases via SDF-1/CXCR4 axis for ASC recruitment and retention.
SDGs
Other Subjects
Biocompatibility; Cytology; Gelation; Hyaluronic acid; Stem cells; Adipose stem cells; Gelation temperature; Injectable hydrogels; Material structure; Therapeutic angiogenesis; Therapeutic strategy; Thermally responsive hydrogels; Thermo-responsive; Hydrogels; chemokine receptor CXCR4; chitosan; stromal cell derived factor 1; cell culture; cell motion; chemistry; cytology; delayed release formulation; drug effect; human; hydrogel; injection; mesenchymal stem cell; mesenchymal stem cell transplantation; metabolism; signal transduction; Cell Movement; Cells, Cultured; Chemokine CXCL12; Chitosan; Delayed-Action Preparations; Humans; Hydrogels; Injections; Mesenchymal Stem Cell Transplantation; Mesenchymal Stem Cells; Receptors, CXCR4; Signal Transduction
Publisher
Royal Society of Chemistry
Type
journal article