Effect of Baicalein on Apoptosis of the Human Hep G2 Cell Line Was Induced by Mitochondria Dysfunction
Resource
PLANTA MEDICA v.68 n.4 pp.302-306
Journal
PLANTA MEDICA
Journal Volume
v.68
Journal Issue
n.4
Pages
302-306
Date Issued
2002
Date
2002
Author(s)
TSAI, CHING-LIN
Abstract
The effects of baicalein on the human hepatoblastoma G2 (Hep G2) cell line were investigated in this study. By an SRB viability assay, we demonstrated that baicalein reduced the viability in a dose- and time- dependent manner. The apoptotic features such as chromatin condensation and DNA fragmentation were observed in the baicalein-treated cells. During the process of apoptosis, we noticed a sequential dissipation of mitochondrial membrane potential (DeltaPsi(m) ) and an apparent redistribution of cytochrome c from the mitochondria to the cytosol in baicalein-treated cells. Furthermore, the mitochondrial Bcl-2 protein represented a dramatic change in response to baicalein treatment. Altogether, our data suggested that the effect of baicalein on apoptosis of the human Hep G2 cell line was induced by mitochondrial dysfunction and Bcl-2 regulation.
Subjects
Scutellaria baicalensis
Labiatae
baicalein
apoptosis
mitochondrial membrane potential
cytochrome c