副甲狀腺素對腎小管鈉氫交換器及重碳酸鈉共同運送器的調節
Date Issued
2003
Date
2003
Author(s)
朱宗信
DOI
912314B002350
Abstract
Parathyroid hormone（PTH）decreases phosphate, sodium and bicarbonate reabsorption by the proximal tubule. Proximal tubule transcellular NaCl and NaHCO3 reabsorption is mediated by an apical membrane Na/H exchanger,（NHE-3）and a basolateral Na/HCO3（NBC-1）.OKP cells, a cell line of renal proximal tubule, express NHE-3 . The regulating factors of NHE-3 are multiple. Acid incubation increases NHE-3 activity, NHE-3 mRNA abundance, NHE-3 protein abundance. PTH
acutely inhibits NHE-3 in OKP cells via a dual signaling cascade involving protein kinase A and C. The purpose of this project is to study the chronic effect of PTH on NHE-3 activity, NHE-3 protein abundance.
We used OKP cell as the experiment model. Continuous measurement of intracellular pH was accomplished in cells using pH-sensitive dye
BCECF. Na/H exchanger activity was assayed as the initial rate of Na-dependent pHi recovery from an acid load. We checked chronic effect
of PTH in different concentration on Na/H exchange activity in renal tubular cell. We checked the effect of PTH on NHE-3 protein abundance
in OKP cell.
The results showed that there was no significant change of NHE-3 activity or NHE-3 protein abundance by PTH in OKP cells. We think the major effect of PTH is for Ca & P, not for Na & acid.
Subjects
parathyroid hormone
renal tubule
Na/H exchanger
Publisher
臺北市：國立臺灣大學醫學院一般醫學科
Type
journal article
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