花生簇葉病病原菌質體gyrB和gyrA基因之選殖與分析(1/2)
Date Issued
2003
Date
2003
Author(s)
林長平
DOI
912313B002336
Abstract
DNA gyrase is a type II topoisomerase that is a tetrameric molecule composed of
two A and two B subunits, which are encoded by the gyrA and gyrB genes,
respectively. In order to clone and analyses the gyrB gene, a pair of
oligo-nucleotides PCR primer GBF2/ GBR3 was designed according to the sequences
of the gyrB gene of Acholeplasma laidlawii, Escherichia coli, Bacillus sp.,
Staphylococcus aureus, Streptococcus pneumoniae, Streptomyces spheroides,
Spiroplasma citri and Mycoplasma spp. Total DNA from diseased periwinkle
infected with phytoplasma associated with peanut witches’ broom （PNWB）was
prepared for PCR reaction to amplify a 1458 bp-PCR fragment of the gyrB gene of
the phytoplasma. A 768 bp-PCR product was then amplified by using the primers
GBF3, GBR5 synthesized according to the sequences of the 1458 bp-PCR fragment
and used as a nucleic acid probe for the screening of the Lambda ZAP II genomic
library of PNWB-phytoplasma.
Subjects
gyrB gene
gyrA gene
peanut witches broom
phytoplasma
Publisher
臺北市：國立臺灣大學植物病理與微生物學系暨研究所
Type
other
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