Analysis of Gene Expression and Function in Rice Molybdenum Cofactor Sulfurase-like Gene
Date Issued
2007
Date
2007
Author(s)
Huang, Ping-Min
DOI
zh-TW
Abstract
ABA is considered as an important stress hormone. The last step of ABA biosynthetic pathway is catalyzed by aldehyde oxidase (AO). The combination of AO with a sulfurized molybdenum cofactor (Mo-cofactor) is necessary for enzyme activity. Therefore Mo-cofactor sulfurase (MCSU), the enzyme in charge of the process of Mo-cofactor sulfuration, is one of the important factor for controlling of ABA biosynthesis. Base on the search on rice genome sequence, three molybdenum cofactor sulfurase-like (MCSUL) genes, named OsMCSUL1, OsMCSUL2 and OsMCSUL3, are presented on rice genome. The putative OsMCSUL3 amino acid sequence shared approximate 60％ homology with Arabidopsis MCSU. According to the protein functional domain analysis, the N-terminal end of OsMCSUL3 contains a putative Nifs domain. The C-terminal end of OsMCSUL3 is the MCSU conserved region. On the other hand although there is no Nifs function domain found on OsMCSUL1 and OsMCSUL2, the full length of Osmcsul1 and OsMCSUL2 have high homology with C-terminal of Arabidopsis MCSU. In this study, an effort was made to determine gene expression of OsMCSUL family genes in salt-stress rice seedling. Base on real-time RT-PCR analysis OsMCSUL1 expression was up-regulated by salt stress and ABA in both leaf and root tissues. OsMCSUL2 gene expression almost could not be affected by NaCl in leaves and roots, however, it’s expression was enhanced by ABA. OsMCSUL3 mRNA level was in salt-stress root samples, but not in leaves. In addition, ABA was a positive on regulator for OsMCSUL3 expression in roots, but not in leaves. To determine the function of OsMCSUL genes, the genes were transformed into Arabidopsis mcsu mutant (aba3 mutant), the result suggested that OsMCSUL2 was likely to share identical (or similar) functions of the protein of the Arabidopsis MCSU, which can active AO than ABA biosynthesis.
Subjects
離層酸
水稻類鉬輔因子硫化酶
基因
ABA
OsMCSUL gene
Type
thesis
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