Factors Influenced Candida Albicans Adhesion on Denture Surface
Date Issued
2009
Date
2009
Author(s)
Hung, Chia-Ling
Abstract
Candida albicans was the most common yeast species found in denture plaque of patients with denture stomatitis. Regarding to Candida albicans adhesion on denture surface, many studies had focused on the influences of physical characteristics of acrylic resin, such as surface roughness, surface free energy or resin materials, etc… The denture fabrication process is complicated which may also play an important role in Candida adhesion. Moreover, denture is soaked in saliva all the time during wearing. Saliva is viscous and contains enzymes which may complicate the adhesion of Candida on denture surface. Seldom studies focused on factors of denture fabrication procedures and saliva together to evaluate the adhesion of Candida albicans on denture surface.he aim of this in vitro study was to evaluate the relationship between factors that may influence adhesion of Candida albicans on denture surface, such as denture surface roughness, separating fluid used in denture processing and human saliva.aterials and methods: 148 pieces of heat-cured acrylic resin (Luciton 199), 20x50x2mm in size, were fabricated by standard procedure. Saliva was obtained from 5 different health people. The specimens were randomly separated into 8 groups which were treated with three different factors during resin plate fabrication procedure: with- or without separating fluid, with or without polishment procedure, immerse in saliva or not. Each group has its negative control. The surface roughness parameters (Ra) of all resin plates were measured by Surftest4 before the study. After cleaning and disinfection, all samples were placed into Candida albicans SC5314 broth with concentration of 105 cell/ml. The test tube was incubated for 1 hour at 37oC in an orbital shaker to promote yeast adherence. On hour later, all samples were air dried and imprinted on SDA agar for half hour. Then the resin plates were removed and agar was incubated at 37oC for 18 hours. Colony forming unit (CFU) of Candida Albicans on the agar surface were counted and the results were analyzed by correlation analysis, multiple regression analysis & ANOVA.esult:he results showed that surface roughness in this study was not a significant factor influenced the Candida albicans adhesion on denture surface. (P=0.562). Using separating fluid during acrylic resin processing was significant which increased Candida adhesion (P=0.008) Human saliva was also showed to be a significant factor but would decrease the Candida adhesion (P=0.001), There was no interaction among these experiment factors. onclusion: It can be concluded that denture separating fluid and human saliva showed influences on Candida adhesion, the former is positive, and the later is negative.
Subjects
Candida adhesion
Surface roughness
saliva
denture separating fluid
SDGs
File(s)![Thumbnail Image]()
Loading...
Name
ntu-98-R94422021-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):bed7cfef98960df202451447a7ea8e0b