Structure and functions of γ-dodecalactone isolated from Antrodia camphorata
Date Issued
2008
Date
2008
Author(s)
Chen, Chia-Jung
Abstract
In Taiwan, Antrodia camphorata (A. camphorata) is a traditional Chinese medicine and it has been utilized to treat a wide variety of diseases. However, the immuno-activating function of the flavor components from A. camphorata had not yet been investigated. In the present work, the volatile oil of A. camphorata was analyzed with the aim of isolating and identifying the major flavor compound and investigating the relationship between the chemical structure and biological functions of compound. The major component of A. camphorata volatile oil was identified as gamma-dodecalactone (gamma-DDL) by GC/MS analysis. By GC and flow cytomertry analysis, suggested that gamma-DDL entered the cells for the induction of human umbilical cord blood mononuclear cells (hUCB-MNCs) phenotypic changes and activation of NK cells. gamma-DDL was found to convert its open ring form: 4-hydroxydodecanoic acid for the activation of NK cells. The active configuration of gamma-DDL was deduced with the help of chiral derivatising agents (CDAs) and HPLC analysis. Only the R-configurated form of gamma-DDL was able to mediate NK cell activation. Furthermore, the configuration of gamma-DDL was identified in A. camphorata also as R-form. Finally, gamma-DDL induced rapid activation of interferon-2 (IL-2) gene in NK cells and the IL-2 autocrine system to activate NK cells. gamma-DDL also induced the activation of T cells, B cells, monocytes and DCs, but there might be different mechanisms of activation.
Subjects
γ-DDL
Antrodia camphorata
hUCB-MNCs
NK cells
active configuration
IL-2 autocrine system
SDGs
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