B型肝炎病毒基因型與DNA濃度的家族相關分析
Familial correlation of hepatitis B virus genotype and DNA loads
Date Issued
2005
Date
2005
Author(s)
Li, Yi-Hsiu
DOI
zh-TW
Abstract
Background Hepatitis B virus (HBV) genotype and DNA levels have been associated with hepatocellular carcinoma (HCC) risk. The aims of this study were to investigate familial correlations for the two viral factors, and to assess whether these factors may influence the degree of familial aggregation of HCC.
Materials and Methods A total of 766 HBV surface antigen-positive individuals were included. These individuals were from 280 families ascertained through a HCC proband, including 210 simplex and 70 multiplex families (defined as having an additional patient with HCC among first-degree relatives of the proband). We used real-time polymerase chain reaction assays of plasma DNA samples to quantify HBV DNA levels and determine HBV genotype for all subjects. Multiple linear regression was used to assess correlations between various factors and HBV DNA levels. Unconditional logistic regression was used to estimate sibling concordant odds ratios and their 95% confidence intervals (CIs) for HBV DNA levels. Familial correlation was calculated with use of the uniform weighting scheme as implemented in the FCOR program of SAGE version 5.0.
Results For 247 (88.2%) of the 280 families, all family members were infected with the same HBV genotype. The prevalence of genotype C HBV was higher in affected than in unaffected individuals; this difference was statistically significant in multiplex families (P=0.05). Multiplex families had a higher prevalence of genotype C than simplex families. Gender (P=0.048), HBV genotype (P=0.003), and HCC status (P=0.020) were significantly associated with HBV DNA levels. After adjusting for gender, HBV genotype, and HCC status, HBV DNA levels showed higher parent-offspring (r=0.23, P=0.008) and lower sibling correlations (r=0.14, P=0.020). Particularly, the mother-son correlation coefficient reached 0.37 (P=0.001). When 6.46 log copies/mL was used as the cutoff point to classify the data, we obtained a sibling concordant odds ratio of 2.12 (95% CI=1.12-6.93) for all sib pairs (including affected-unaffected discordant pairs), and 2.90 (95% CI=1.21-6.93) for unaffected sib pairs.
Conclusions We found strong familial correlation for HBV genotype. There were significant parent-offspring and sibling correlations for HBV DNA levels. Genotype C was associated with the degree of familial aggregation of HCC.
Subjects
B型肝炎
肝細胞癌
基因型
DNA濃度
家族相關分析
HBV
genotype
viral load
familial correlation
HCC
SDGs
Type
thesis
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