Development of High Amylose Maize with a Cross Incompatibility Gene by Marker-Assisted Selection
Date Issued
2015
Date
2015
Author(s)
Chen, Yu-Ru
Abstract
Maize (Zea mays L.) is one of the most important cereals in the world and is beingconsidered as a prominent source for starch. Its starch plays a significant role in our daily life. Especially, its amylose content is not only advantageous to human as a food but also can be applied in industry. In maize, only 20-30% of starch is amylose; however, the amylose content can be reached to 50% resultedby anae recessive gene, which is called high amylose maize.. If planted high amylose maize in fields without proper isolation, the quality of seeds might be affected by xenia through pollen pollutants. This study is aimed to introduce Ga1-S conferring cross-incompatibility into high amylose maize to insure its quality. Plants with Ga1-Scan greatly reduce the probability of cross-pollination; nevertheless, the pollination successeswhile pollens and pistils have the same genotype. Two backcross populations were developed herein thatby popcorn HP301 and HP68-07 with Ga1-S, as the donor parents, crossed with GEMS-0067,with high amylose content, as the recurrent parent. The two indel markers flanking Ga1-s were applied to marker-assisted selection in the two backcross populations. Throughfour seasons of backcrossing, the two populations are now reached to BC3F1 generation. In addition, Ga1-S and genes corresponding to amylose content, including major gene ae and minor gene SbeI, were introduced into 172 maize inbreds to developthe germplasm for selecting and breeding hybrids of high amylose maize.
Subjects
cross-incompatibility
high amylose maize
marker-assisted selection
Type
thesis
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