New protein PCiP from edible golden oyster mushroom (Pleurotus citrinopileatus) activating murine macrophages and splenocytes
Journal
Journal of the Science of Food and Agriculture
Journal Volume
87
Journal Issue
8
Pages
1550-1558
Date Issued
2007
Author(s)
Abstract
A new immunomodulatory protein (PCiP) was purified from an edible golden oyster mushroom (Pleurotus citrinopileatus) by extraction with 5% (v/v) cold acetic acid in the presence of 0.1% (v/v) 2-mercaptoethanol, followed by ammonium sulfate fractionation, DE-52 and MonoQ anion-exchange chromatography. Electrophoresis assays demonstrated that the molecular mass of PCiP was approximately 15.0 kDa and its pI was around 5.2. PCiP is a simple protein without carbohydrate, and cannot agglutinate mouse red blood cells, suggesting PCiP is not a lectin. In addition, PCiP (5-20 μg mL-1) alone activated murine splenocytes, and markedly increased their proliferation and gamma-interferon (IFN-γ secretion, but suppressed MTT metabolization, while murine splenocytes were simultaneously stimulated by the mitogen concanavalin A (ConA). Furthermore, PCiP (5-20 μg mL-1) directly activated murine macrophages and increased the production of both the nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α by RAW 264.7 macrophages. These findings suggest that PCiP could strengthen both the innate and adaptive responses of its host. © 2007 Society of Chemical Industry.
SDGs
Type
journal article