The Effects of Secretory Lcn2 Protein on Mouse Primary Endometrial Epithelium.
Date Issued
2010
Date
2010
Author(s)
Chen, Yan-Tzu
Abstract
Lcn2, a member of the lipocalin family, is as a secretory protein in mammalian system as well as has autocrine and paracrine physical functions. A well known, Lcn2 is an acute phase protein and is induced by stress conditions in cells. Owing to the stress, epithelial to mesenchymal transition (EMT) could occur and the substantial evidences encourage us to figure out the relationship between Lcn2 and EMT in primary endometrial epithelium cell. To establish primary culture system of mouse endometrial epithelium cell is our matter of immediate urgency. According to the method of Glasser et al, after trypsin digestion, we haversted primary endometrial epithelium cells and the cells have been identified with cytokeratin antibody. Using nutrition deprivation as a stress condition, we could induce highly Lcn2 expression in this endometrial epithelium cell. To elucidate whether Lcn2 triggers the EMT in endometrial epithelium cell then initiates the influence of the cell function in reproductive system is our goal. As the Lcn2 expression in high level, the cell morphology would change from round-up to spindle-like then the cell dispersion would happen. Meanwhile, double immunostaining showed cytokeratin expression was diminished and fibronectin expression was increased, and an additive effects on cell migration and cell invasion. These results demonstrate that Lcn2 could be induced by environmental stress and trigger the EMT in primary mouse endometrial epithelium cell. These data shield some light on the participation of Lcn2 in the pathologies of endometrial system.
Subjects
mouse endometrial epithelium
epithelial to mesenchymal transition (EMT)
cytokeratin
fibronectin
Type
thesis
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