利用超音波來進行體外基因轉移之研究
Date Issued
2004-07-31
Date
2004-07-31
Author(s)
陳文翔
DOI
922312B002019
Abstract
It is always a great challenge to develop a safe and effective method for
delivering gene into cells. The main purpose of this study is to determine
optimal acoustic parameters for effective gene transfer and high cell survival in
the ultrasound-mediated gene transfection. The parameters we studied
included the pulse repetition frequency (PRF), the length off time and the ratio
of on to off time (on-off ratio). We found that for cultured cervical cancer cells
(HeLa cells), the best PRF for gene transfection ranges between 2 and 2000
Hz (50% duty cycle). The transfection efficiency declined rapidly while PRF
was higher than 1000Hz and dropped to 1% while PRF was 4000 Hz. When
the ultrasound pulse repetition frequency (PRF) was shorter than 2 Hz (the
exposure on-time was larger than 250-ms), the transfection efficiency was high,
but the extremely high temperature elevation resulted in low cell survival.
While PRF was between 20 Hz to 1000 Hz, a better transfection could be
obtained when the ratio of the on-time to the off time was larger than 0.1,
i.e. > 0.1
−
−
off time
on time . When the ratio was smaller than 0.1, the transfection
efficiency was close to 0%.
Subjects
ultrasound
gene therapy
gene transfer
gene
transfection
SDGs
Publisher
臺北市：國立臺灣大學醫學院復健科
Type
other
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