Effects of red mold dioscorea on oral carcinogenesis in DMBA-induced hamster animal model
Resource
FOOD AND CHEMICAL TOXICOLOGY, 49(6), 1292-1297
Journal
Food and Chemical Toxicology
Journal Volume
49
Journal Issue
6
Pages
1292-1297
Date Issued
2011
Author(s)
Abstract
Monascus-fermented products offer valuable therapeutic benefits and have been extensively used for centuries in East Asia. Dioscorea has been proved to have anti-cancer effect. The aim of this study is to investigate the anti-tumor ability of the ethanol extract of red mold dioscorea (RMDE) on 7,12-dimethyl-1,2-benz[a]anthracene (DMBA)-induced hamster buccal pouch carcinogenesis. We induced oral squamous cell carcinoma (OSCC) in the buccal pouch of male Syrian golden hamsters by painting with 0.5% DMBA three times a week for 14weeks. From 9 to 14weeks, a dose of 50, 100, and 200mg RMDE per kg body weight were painting with the hamsters for 6weeks on days alternate to the DMBA application. The results demonstrated that RMDE decreased nitric oxide (NO), reactive oxygen species (ROS), and prostaglandin E2 (PGE2) overexpression in hamster buccal pouches in the DMBA treatment group and increased p53, serum tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) to significantly stimulate caspase-8 and -3 activities, indicating that RMDE reduced oxidative damage causing by DMBA and induced apoptosis in oral cancer cells. Therefore, RMDE may have therapeutic potentials against OSCC. ? 2011 Elsevier Ltd.
Subjects
7,12-Dimethyl-1,2-benz[a]anthracene (DMBA); Ethanol extract of red mold dioscorea (RMDE); Hamster buccal pouch (HBP); Monascus-fermented products; Oral cancer; Oral squamous cell carcinoma (OSCC)
SDGs
Other Subjects
alcohol; antineoplastic agent; caspase 3; caspase 8; caspase 9; cyclin B1; dimethylbenz[a]anthracene; interleukin 1beta; nitric oxide; plant extract; prostaglandin E2; protein p53; reactive oxygen metabolite; red mold dioscorea extract; tumor necrosis factor alpha; unclassified drug; animal experiment; animal model; animal tissue; antineoplastic activity; apoptosis; article; carcinogenesis; cheek pouch; controlled study; dose response; drug dose comparison; enzyme activation; enzyme activity; gene overexpression; male; mouth carcinoma; nonhuman; oxidative stress; protein blood level; red mold dioscorea; squamous cell carcinoma; Syrian hamster; toxicity testing; treatment duration; yam; 9,10-Dimethyl-1,2-benzanthracene; Animals; Antineoplastic Agents, Phytogenic; Antioxidants; Carcinogens; Carcinoma, Squamous Cell; Cricetinae; Dinoprostone; Dioscorea; Disease Models, Animal; Male; Mesocricetus; Monascus; Mouth; Mouth Neoplasms; Nitric Oxide; Plant Extracts; Reactive Oxygen Species; Animalia; Cricetinae; Dioscorea; Mesocricetus auratus; Monascus
Type
journal article