Effect of light and cultivation method on growth and volatile compounds of green onion (Allium fistulosum L.)
Date Issued
2012
Date
2012
Author(s)
Wu, Hung-Chun
Abstract
The purpose of this study is to investigate the growing of a local strain-Black leaf, a variety of green onion called Shy-Zih, They were planted in the growth chambers for fogoponic and hydroponic culture systems. Three kinds of light qualities are used as artificial light source. Additionally, the solid-phase microextraction (SPME) and gas chromatography - mass spectrometry (GC-MS) were used to analyze the volatile components from the leaves and roots.
First is to establish the proper light intensity, the measured light saturation point of the “Black leaf” is 382.15 ± 27.8μmol．m-2．s-1 (equivalent to 24,993.46 ± 1,818 lux) if the initial concentration of carbon dioxide was at 1,500±43 ppm; when the initial concentration of carbon dioxide increased at 3,000±71pp, the light saturation point is greater than 711μmol．m-2．s-1.
No significant difference in the green-leaf growth rate (green-leaf surface area) by fogoponic and hydroponic culture systems. But the average of rooting number and growth rate with fogoponic cultivation is better.
Under the white, red and blue light, the average growth number of leaf and surface area of “Black leaf” it makes significant difference among them and it grows best under the white light. The analyzing condition of the volatile components of fresh leaves is to heat temperature up to 75 ℃ and SPME heating adsorption for 7 minutes.
The Dipropyl disulfide、1,2-Dithiolane and Dipropyl trisulfide of green-leaf of ''Black Leaf” contents is higher than it grown under red and blue light. The 3,5-Diethyl-1,2,4-trithiolane contents is higher than it grown under other two kinds light and the volatile compounds are the lowest among all.
The roots of the “Black-leaf” content more volatile compounds than the green-leaf. If the roots are grown under the blue light, the 5 kinds of volatile compounds(Methyl 2-propenyl disulfide、Dimethyl trisulfide,(Z)-1-(methylthio)-1-propene、(E)-1-(methylthio)-1-propene、3-Isopropyl-4-methyl-dec-1-en-4-ol) content are higher than it grown under red and blue light and the difference is very significant. Besides, compare with the roots grown in greenhouse, the 3 kinds of volatile compounds are higher than it was grown under the natural light. The 5 kinds of volatile compounds of roots, Methyl propyl disulfide、Dipropyl disulfide、3,3-Dimethyl-piperidine、2-[(methylthio)ethynyl]-thiophene and 3,5-Diethyl-1,2,4-trithiolane, are higher than the green-leaf but the 1,2-Dithiolane is opposite.
Subjects
indoor cultivation
fogoponic
light quality
light intensity
SPME
GC-MS
Type
thesis
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