Cellular biodistribution of polymeric nanoparticles in the immune system
Journal
Journal of Controlled Release
Journal Volume
227
Pages
82-93
Date Issued
2016
Author(s)
YA-WUN YANG
Luo W.-H.
Abstract
The biodistribution of polymeric nanoparticles (NPs) is of crucial importance in the development of nanoparticle-based vaccine delivery or immunotherapy for cancer. The purpose of this study was to investigate the kinetics of cellular biodistribution of polymeric NPs in the immune system. Polystyrene (PS) yellow-green nanoparticles (YG-NPs) 500 nm in diameter were intravenously (i.v.) injected into the tail veins of mice, and the kinetics of YG-NP biodistribution was followed by harvesting cells at pre-determined time points from various immune organs, including blood, bone marrow, spleen, and lymph nodes and analyzing them by polychromatic flow cytometry. To observe the location of YG-NPs in the spleen after i.v. administration, spleens of mice were isolated at 6 h post-injection (p.i.), cryosectioned, immunostained, and examined by confocal microscopy. Our data show that the major phagocytosing cells included granulocytes (B220-CD11b+ Gr-1highLy-6Clow) in the blood and bone marrow and B cells (CD11b-B220+) in the spleen. The kinetics of the phenotypic analysis suggest the potential trans-differentiation of the B220-CD11b+ Gr-1lowLy-6Chigh subset into B220-CD11b+ Gr-1-Ly-6C- double-negative (DN) cells expressing the F4/80 macrophage phenotype in the blood and CD115 in the bone marrow after treatment with YG-NPs. Based on the microscopic analysis of spleen cryosections, the majority of YG-NPs were located in the marginal zones (MZ) and red pulp of the spleen at 6 h post-injection (p.i.), allowing further interaction with MZ macrophages and granulocytes. The data obtained in this study demonstrate the kinetics of biodistribution of polymeric nanoparticles in the blood, bone marrow, and spleen at the cellular level. 2016 Elsevier B.V. All rights reserved.
Subjects
Cellular biodistribution; Immune system; Polymeric nanoparticles
SDGs
Other Subjects
Blood; Bone; Cells; Cytology; Immune system; Kinetics; Macrophages; Mammals; Nanoparticles; Biodistributions; Double negatives; Macrophage phenotypes; Microscopic analysis; Phenotypic analysis; Polymeric nanoparticles; Trans differentiations; Vaccine delivery; Polymers; nanoparticle; polymer; polystyrene; coloring agent; nanoparticle; polystyrene derivative; animal experiment; animal tissue; Article; B lymphocyte; blood; bone marrow; cellular distribution; confocal microscopy; flow cytometry; granulocyte; immune system; lymph node; mouse; nonhuman; phagocyte; priority journal; spleen; animal; C57BL mouse; immune system; metabolism; tissue distribution; ultrastructure; Animals; Bone Marrow; Coloring Agents; Immune System; Lymph Nodes; Mice, Inbred C57BL; Nanoparticles; Polystyrenes; Spleen; Tissue Distribution
Type
journal article