Role of monocarboxylate transporters in the development of central nervous system of zeabrafish (Danio rerio)
Date Issued
2007
Date
2007
Author(s)
Kao, Zhi-Jie
DOI
en-US
Abstract
Astrocytes provide energy to neuron mainly with lactate, which is transported through monocarboxylate transporters (MCTs). Among 14 isoforms of MCTs reported in mammals, only MCT1, 2 and 4 are expressed in brain and the three isoforms are found to differentially expressed in neuron and astrocyte, respectively. Based on these, “astrocyte-neuron lactate shuttle” has been proposed. However, no in vivo evidence was available so far to support this hypothesis.
In the present study, zebrafish was used as a model to provide in vivo molecular physiological evidence for the involvements of MCTs in the development and functioning of central nervous system (CNS). Full-length cDNAs of the zebrafish MCT1-4 were cloned from zebrafish. In RT-PCR and double in situ hybridization/immunocytochemistry experiments, the zMCT1-4 were all expressed in brain of embryos, and were further found to localize in both neuron and astrocyte. Morpholino knockdown experiments provided for the 1st time the in vivo evidence to indicate that the zMCT1-4 may be involved in energy translocation and functioning of the developing brain. Subsequent rescue experiments with mRNAs of specific isoforms further indicated zMCT2 to be an indispensable transporter for CNS development and function.
Subjects
星狀細胞
單羧基運輸蛋白
Astrocyte
monocarboxylate transporter (MCT)
Type
other
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