斜紋夜蛾微粒子蟲之研究: 核糖體, 極管蛋白及孢壁蛋白基因(1/3)
Date Issued
2003
Date
2003
Author(s)
王重雄
DOI
912313B002360
Abstract
The morphologically similar spores of five microsporidian isolates were
collected from five important lepidopteran pests: Spodoptera litura, S. exigua,
Helicoverpa armigera, Plutella xylostella, and Artogeia spp. in Taiwan during
seasonally epizootic microsporidiosis. The isolate from S. exigua had the highest
virulence to third-instar larvae of S. litura. The isolate from P. xylostella had the
lowest virulence but yielded more progeny spores in the infected larvae than other
isolates. This result showed that these isolates have high production in alternate
hosts. The phylogenic relationships of these five isolates were analyzed by
immunological relationship and the homology of the small subunit rDNA sequences.
Based on the patterns of Western-blot hybridization, three serotypes could be
identified. The isolate from P. xylostella is a unique serotype, and the other two
2
serotypes are N. spodopterae/Artogeia spp., and S. exigua/H. armigera. There were
no significant differences in the SSUrDNA sequences, suggesting that they all belong
to the genus Nosema, but they could be distinguished by random amplified
polymorphic DNA polymerase chain reaction (RAPD-PCR). Five commercial
primer sets (OPA, OPAC, OPBD, OPO, and OPU), with each set containing 20
primers, were used for this study. Using each microsporidian DNA with every
primer for PCR amplification, the yields of RAPD-PCR profiles were compared.
Sixty primers were selected and suggested for intra- or interspecies microsporidian
identification and also for phylogenic analysis for these five isolates. Based on the
analysis of the Unweighted Pair-Group Method with Arithmetic Averages (UPGMA)
applying software from the NTSYS-pc v. 2.01b, isolates could be clearly divided into
three groups, the result is coincident with the serotypes. RAPD-PCR analysis of
microsporidian DNA, as shown in these studies, is a readily available, rapid method
for distinguishing and confirming microsporidian species and isolates, especially
those for which ultrastructural studies are lacking. In addition, we used a N.
bombycis specific primer pair to detect our isolates by polymerase chain reaction.
The result implied that the isolate of P. xylostella was closely related with N.
bombycis. This work has been published in Journal of Invertebrate Pathology, 83
（2003）pp. 51-59.
Subjects
microsporidia
Spodoptera litura
Nosema
Publisher
臺北市：國立臺灣大學昆蟲學系暨研究所
Type
report
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