Isolation and Characterization of Seven Tetrarepeat Microsatellite Loci in the Indo-Pacific Finless Porpoise (Neophocaena phocaenoides) and Their Amplification across Cetacean Species
Other Title
七個印太洋露脊鼠海豚（Neophocaena phocaenoides）之四重複核苷酸微衛星DNA基因座選殖與其在鯨豚多樣性研究之應用
Journal
Collection and Research
Journal Issue
33
Start Page
1
End Page
7
ISSN
1726-2038
Date Issued
2020-12
Author(s)
Abstract
The Indo-Pacific finless porpoise (Neophocaena phocaenoides) inhabits a narrow band of shallow waters along the coastal Indo-Pacific and experiences high levels of incidental mortality due to fishery activities. We isolated seven polymorphic microsatellite loci with (GAAA)_n motifs from finless porpoises using an enrichment cloning procedure. We characterized these loci by screening 32 finless porpoises from the Taiwan Strait. The mean number of alleles per locus was 10.3 (range 5~16). The observed heterozygosity of each locus ranged 0.3~0.9. Evaluation of cross-species amplification of these markers across 14 cetaceans in five families indicated that these microsatellite loci are useful for ecological, evolutionary, and conservative research on the finless porpoise, as well as other cetacean species.
印太洋露脊鼠海豚（Neophocaena phocaenoides）分布於印太洋地區的近岸海域，族群生存深受人類對海岸淺海的開發與漁業活動之影響。我們利用濃化選殖法從印太洋露脊鼠海豚DNA中篩選出7個具有多型性的四重複核苷酸微衛星DNA基因座，並檢視這些基因座在32隻露脊鼠海豚的分子特性。結果顯示上述基因座的平均對偶基因數為10.3（各基因座的對偶基因數介於5至16）。各基因座的異型合子歧異度觀察值（observed heterozygosity）介於0.3至0.9之間。此外，我們也檢視這些基因座在其他5科14種鯨豚的可增幅性與多型性。本研究所開發的7個新遺傳標誌未來可應用於不同鯨豚物種的演化生態與保育遺傳等多樣性研究。
Subjects
多型性
polymorphism
印太洋露脊鼠海豚
微衛星DNA基因座
鯨豚
cetacean
Indo-Pacific finless porpoise
microsatellite DNA loci
Publisher
國立自然科學博物館
Type
journal article