Antibacterial Effects of Needle Type Nano-second Pulsed Atmospheric-pressure Cold Plasma on Streptococcus mutans and Enterococcus faecalis
Date Issued
2015
Date
2015
Author(s)
Hsiao, Shun-Chung
Abstract
Oral disease is highly related with microbiological infection. In several decades, treatment of dental infection was accomplished by mechanical preparation and chemical irrigation. However, primary goal of treatment is to decrease the total number of bacteria, but total elimination of bacteria is impossible; In addition, accident may occurred during chemical irrigation and may damage to human tissue such as skin, mucosa, periapical tissue, etc. In recent years, plasma system can operate in atmosphere condition at room temperature and pose a potential to application in dental treatment. In this study, Streptococcus mutans, and Enterococcus faecalis which are important oral pathogens were selected as a target to perform antibacterial test with plasma system. Diameter of inhibition zone was measured in each condition for the purpose of quantitative analysis. In this study, Needle Type Nano-second Pulsed Atmospheric-pressure Cold Plasma system was established and use pure helium and helium with oxygen mixture as two kinds of working gas; Controllable conditions including flow(gas flow rate), time(plasma exposure time), distance(applied distance of plasma). With respect to clinical limitation, all condition was set to a limited range. Result shows: (1)Flow is a determinant factor of antibacterial effect, quantitative study shows diameter increases with flow; but in qualitative study, with increase of flow(higher than 1.0 slm),incomplete bactericidal effect was noted over center zone of inhibition. (2)With fixed condition of flow, linear correlation is significant between time and diameter also significant between distance and diameter. (3)Group of Helium with oxygen mixture: with limited condition of flow(0.5~1.0 Standard Liter per Minute), minimum effective time is 15 seconds.(4)Pure helium group :with limited condition of flow(1.5 Standard Liter per Minute), minimum effective time is 60 seconds (5) No significant difference between two species of bacterial in bactericidal effect(6)With addition of oxygen into Helium, obvious higher bactericidal effects was noted compared with groups of pure helium. In conclusion, this new plasma system has an effective bactericidal effect on oral pathogen within limited conditions which are applicable in clinical treatment. Further study is indicated with biofilm condition and experiment in vivo.
Subjects
Antibacterial
nano-second pulsed power module
Atmospheric-pressure Cold Plasma
Streptococcus mutans
Enterococcus faecalis
SDGs
Type
thesis
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