Effect of Collagen Conformation and Components on Adhesion, Growth and Functions of Osteoblast-Like Cells
Date Issued
2005
Date
2005
Author(s)
Cheng, Yu-Hui
DOI
zh-TW
Abstract
Collagen and glycosaminoglycans are the major components of extracellular matrix, and are important in tissue formation and tissue engineering. This study was proposed to investigate the effects of conformation of type I collagen, and the effects of glycosaminoglycan on adhesion, proliferation and function of human osteosarcoma MG-63. Cells were seeded on TCPS plates coated with soluble collagen, fibrous collagen and heat-denatured collagen, or mixture of collagen and heparin. The attachment, morphology, growth and functions of cells were examined. We found that soluble collagen and fibrous collagen promoted cell attachment and growth more than denatured collagen. Cells cultured on soluble collagen coated plate had higher alkaline phosphatase activity than on the other substrates. In addition, we found that the plate coated with mixture of 0.02 mg/mL heparin and collagen supported better cell adhesion than the substrates coated with higher heparin concentration.
Subjects
膠原蛋白
骨母細胞
細胞貼附
鹼性磷酸酶
肝素
Collagen
osteoblasts
cell adhesion
Alkaline Phosphatase
heparin
Type
thesis
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