以標籤蛋白吸附純化法研究與D型肝炎抗原作用之細胞蛋白
Date Issued
2003
Date
2003
Author(s)
李嘉哲
DOI
912315B002011
Abstract
Hepatitis delta virus (HDV)encodes two isoforms of delta antigens
(HDAgs).The small form of HDAg (SHDAg)is required for HDV RNA
replication,while the large form of HDAg (LHDAg),with additional C-
terminal 19 amino acids,inhibits the viral replication and is
required for viral assembly.Although HDAg was found to bind to RNA
polymerase II,nucleolar phosphoprotein B23,and nucleolin and
modulate HDV RNA replication,the detail mechanism and the role of
HDAg in viral replication remain to be elucidated.Most protein
interaction has been studied with yeast two-hybrid assay,which only
detects interaction between two proteins.Recently,tagged-protein
purification combined with mass spectrometry was applied successfully
to characterize hundreds of distinct multiprotein complexes in the
yeast.We had tried several tagged-vector and found pCMV-TAP,which
contains proteinA and calmodulin was the best.we had found some
proteins,which are characterized by mass spectrometry.
Subjects
delta antigen
tagged-protein affinity purification
mass
spectrometry
spectrometry
SDGs
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
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