MOLECULAR EPIDEMIOLOGY OF PLASMA ONCOPROTEINS IN VINYL CHLORIDE MONOMER WORKERS IN TAIWAN
Resource
CANCER DETECTION AND PREVENTION v.27 n.2 pp.94-101
Journal
CANCER DETECTION AND PREVENTION
Journal Volume
v.27
Journal Issue
n.2
Pages
94-101
Date Issued
2003
Date
2003
Author(s)
LUO, JIIN-CHYUAN
CHENG, TSUN-JEN
Abstract
Aims: To determine the presence of Asp13-p21-ki-ras oncoprotein and p53 oncoprotein in the plasma of vinyl chloride monomer (VCM)-workers in Taiwan. Methods: We used enhanced chemiluminescence (ECL) western blotting to detect Asp13-p21-ki-ras and ELISA to detect mutant p53 protein (p53 -Ag) and anti-p53 antibodies (p53-Ab) in the plasma of VCM- exposed workers. Results: Twenty-five out of 251 (10%) VCM- workers were positive for Asp 13-p21-ki-ras in plasma, but 0 out of 36 controls were positive. There were 15 out of 95 ( 15.8%) plasma-positives among the more highly exposed (> 480 ppm-month) workers and 10 out of 156 (6.4%) plasma- positives among the lesser exposed (less than or equal to480 ppm- month). Compared to the unexposed controls, age and drinking adjusted odds ratios (95% Cl) were 1.2 (0.1, 9.8) in the lower exposed workers, and 4.8 (0.8, 28) in the higher exposed workers, and there was a significant linear trend between exposure and plasma positivity (P = 0.001). Thirty- three out of 251 (13.2%) VCM-workers were positive for the p 53 over-expression (10% with positive p53-Ag and 2 .8% with positive p53-Ab). There was a significant association between cumulative VCM exposure concentration and positive p 53 expression (P = 0.032) among VCM-workers after adjusting for age, hepatitis, drinking and smoking status. Conclusions: Asp I 3-p21-ki-ras oncoprotein and p53 over-expression (p 53-Ag or p53-Ab) can be found in the plasma of VCM-workers in Taiwan, and a significant dose- response relationship exists between plasma oncoproteins expression and VCM exposure. (C) 2003 International Society for Preventive Oncology. Published by Elsevier Science Ltd. All rights reserved.
Subjects
P53 PROTEIN
CHEMICAL CARCINOGENESIS
SERUM ONCOPROTEINS
LUNG- CANCER
MUTATIONS
EXPRESSION
SDGs
Type
journal article