小鼠卵母細胞及早期胚發生時母源性及胚源性基因之表現及其扮演之生物功能(2/3)
Date Issued
2003
Date
2003
Author(s)
鄭登貴
DOI
912313B002374
Abstract
The mZFG-1 gene (mouse zinc finger gene-1; Gene bank accession number:
AF204776) that was first identified from early mouse embryos using the DNA
sequencing shotgun strategy coupled with in silico mining of mouse EST database.
Using the mouse EST sequence-based primer sets for RT-PCR, the mZFG-1 mRNA
was confirmed to be expressed in mouse pre-implantation embryo and in reproductive
organs of adult mice including uterus, ovary and testis. By further library screening
using this cDNA fragment as a probe and 5’-RACE techniques, two (short and long)
4
forms of the mZFG-1cDNAs were obtained. Since both mRNAs encode proteins with
interspersed zinc finger motifs, we refer to the short and long mZFG-1 transcripts as
mZFG-1S and mZFG-1L, respectively. RT-PCR and Northern blotting nanlyses
demonstrated the mZFG-1L mRNA was expressed only in adult testis. The short form
cDNA was ubiquitously found in male and female organs and early pre-implantation
embryos. Localization of these transcripts using in situ hybridization further
confirmed that expression of mZFG-1L occurred only in Sertoli cells; However,
mRNA of mZFG-1S was found in Sertoli and Leydig’s cells of testis and in Granulosa
cells of ovary.
Subjects
mouse embryo
maternal and zygotic gene
differential expression
Publisher
臺北市：國立臺灣大學動物科學技術學系暨研究所
Type
report
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