Effect of the fractions and sub-fractions of adlay seed ethanolic extracts on the growth of human cancer cells
Date Issued
2007
Date
2007
Author(s)
Lin, Jing-Hui
DOI
zh-TW
Abstract
A number of recent studies have shown the physiological activity in anti-tumor effects of adlay extracts. The study was to investigate the effect of fractions and sub-fractions of adlay seed ethanolic extract on the human liver (Hep 3B and Hep G2), breast (MCF7), stomach (AGS) and cervical (HeLa) cancer cell lines and their possible mechanisms. The ethanolic extracts from different parts of adlay seed were named as AHE (hull), ATE (testa), ABE (bran) and PAE (polished adlay). Based on the degree of polarity, these ethanolic extracts (AHE, ATE, ABE and PAE) were separated to hexane layer (He), ethyl acetate layer (Ea), 1-butanol layer (Bu) and water layer (Wa). The results of 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl- tetrazolium bromide (MTT) assay showed that AHE-Ea and ATE-Ea had the significant capacity against growth of all cancer cells and in the dose-dependent manner, so further partitioned into their sub-fractions via column chromatography. The results showed that AHE-Ea-K, AHE-Ea-L, ATE-Ea-E and ATE-Ea-F sub-fractions inhibited the cell viability of gynecological cancer cell lines (MCF7 and HeLa) for 72 hr, and their IC50 values were 13.7-58.2 μg/mL. The results of cell cycle analysis showed that AHE-Ea-K, AHE-Ea-L, ATE-Ea-E and ATE-Ea-F induced MCF7 cell cycle arrest in G0/G1 phase. Furthermore, cell cycle of HeLa arrest in S phase by AHE-Ea-K and AHE-Ea-L, but ATE-Ea-E and ATE-Ea-F induced its cell cycle arrest in G0/G1 phase. According to the results of flow cytometric analysis, it indicated that AHE-Ea-K, AHE-Ea-L, ATE-Ea-E and ATE-Ea-F induced MCF7 and HeLa apoptosis through induction of caspase-3 activity. In conclusion, AHE-Ea-K, AHE-Ea-L, ATE-Ea-E and ATE-Ea-F could be through inducing apoptosis of MCF7 and HeLa cell lines to inhibit their viability.
Subjects
薏苡籽實
人類乳癌細胞MCF7
人類子宮頸癌細胞HeLa
半胱胺酸蛋白酶
-3
細胞凋亡
MTT分析法
細胞週期
adlay seed
human breast cancer cell line MCF7
human cervical cancer cell line HeLa
caspase-3
apoptosis
MTT assay
cell cycle
SDGs
Type
thesis
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