Pigment epithelium-derived factor protects retinal pigment epithelium from oxidant-mediated barrier dysfunction
Resource
BIOINFORMATICS 22 (6): 685-691 MAR 15 2006
Journal
BIOINFORMATICS
Journal Volume
22
Journal Issue
15
Pages
-
Date Issued
2006
Date
2006
Author(s)
DOI
246246/2006111501265150
Abstract
Retinal pigment epithelium (RPE) cells form a monolayer at the blood–retina barrier between the retina and choriocapillaries. The
barrier function may be damaged by multiple stresses to the cell, including the repeated exposure to oxidants that are generated by photoreceptor
cell turnover. The purpose of our study was to document the protective effect of pigment epithelium-derived factor (PEDF), a
tropic factor produced by the RPE, on H2O2-induced RPE barrier dysfunction. When assayed by a FITC-labeled dextran transepithelial
flux, the increased permeability of the RPE barrier (induced by H2O2) was prevented by PEDF pretreatment. To further explore the
mechanism leading to this permeability change, we investigated the distribution of cytoskeleton and junctional proteins. The redistribution
of the two junctional proteins occludin, and N-cadherin and actin reorganization in RPE, induced by H2O2, can be prevented by
PEDF pretreatment. PEDF can also prevent H2O2-induced stress kinase p38/27-kDa heat shock protein signaling which is known to
mediate actin rearrangement. These findings indicated that PEDF can stabilize actin, maintain normal membrane occludin and
N-cadherin structure, and preserve the barrier function of RPE cells against oxidative stress.
Subjects
PEDF
RPE
H2O2
HSP27
p38
Actin
Occludin
N-cadherin
Publisher
Taipei:National Taiwan University Dept Comp Sci Informat Engn
Type
other
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