The interaction between Pseudomonas C27 and Thiobacillus denitrificans in the integrated autotrophic and heterotrophic denitrification process
Journal
Science of the Total Environment
Journal Volume
811
Date Issued
2022
Author(s)
Abstract
Compared to autotrophic and heterotrophic denitrification process, the integrated autotrophic and heterotrophic denitrification (IAHD) shows wider foreground of applications in the actual wastewaters with organic carbon, nitrogen and sulfur co-existing. The efficient co-removal of sulfur, nitrogen, and carbon in the IAHD system is guaranteed by the interaction between heterotrophic and autotrophic denitrificans. In order to further explore the interaction between functional bacteria, Pseudomonas C27 and Thiobacillus denitrifcans were selected as typical heterotrophic and autotrophic bacteria, and their characteristics metabolic responses to different sulfide concentrations were studied. Pseudomonas C27 had higher metabolic activity than T. denitrificans in the IAHD medium with sulfide concentration of 3.12–15.62 mmol/L. Moreover, the fastest sulfide removal rate (0.35 mmol/L·h) was achieved with a single inoculation of Pseudomonas C27. Meanwhile, in mixed inoculant conditions, the interaction between Pseudomonas C27 and T. denitrificans (P:T = 3:1, P:T = 1:1 and P:T = 1:3) yielded the highest sulfide removal efficiency (more than 85%) when sulfide concentration was 6.25–12.5 mmol/L. Additionally, the sulfide removal rate increased with the inoculation proportion of Pseudomonas C27. Thus, this apparent interaction provided a theoretical basis for further understanding and guidance on the efficient operation of IAHD system. ? 2021 Elsevier B.V.
Subjects
Integrated autotrophic and heterotrophic denitrification
Microbial interaction
Pseudomonas
Sulfide
Thiobacillus
Denitrification
Metabolism
Nitrogen
Nitrogen removal
Organic carbon
Sulfur
Sulfur compounds
Autotrophic denitrification
Denitrification process
Heterotrophic denitrification
Microbial interactions
Removal rate
Sulfide concentration
Sulphide
Sulphide removals
Bacteria
acetic acid
carbon
nitrate
nitrogen
sulfide
sulfur
nitric acid derivative
bacterium
concentration (composition)
denitrification
microbiology
wastewater treatment
Article
bacterial growth
bacterial metabolism
coculture
concentration (parameter)
controlled study
degradation
inoculation
integrated autotrophic and heterotrophic denitrification
microbe-microbe interaction
microbial interaction
nonhuman
Pseudomonas C27
Thiobacillus denitrificans
waste component removal
bioreactor
Bioreactors
Nitrates
SDGs
Type
journal article