Urinary biomarkers of oxidative and nitrosative stress and the risk for incident stroke: A nested case-control study from a community-based cohort
Journal
International Journal of Cardiology
Journal Volume
183
Pages
214-220
Date Issued
2015-03-15
Author(s)
Chen, Shu-Ting
Wu, Hon-Yen
Hsu, Hsiu-Ching
Lee, Yuan-Teh
Abstract
Abstract Background Oxidative and nitrosative stress has suggested to be involved in the pathophysiology of cardiovascular diseases, but has unclear relationship with the risk for incident stroke. Methods In this nested case-control study, cases consisted of 131 participants who were free of stroke at screening and experienced incident stroke during the follow-up period. Controls were 1:1 frequency-matched for age and sex. Baseline levels of urinary creatinine-indexed biomarkers were measured using liquid chromatography-tandem mass spectrometry, including 8-iso-prostaglandin F2α (8-iso-PGF2α), 4-hydroxynonenal conjugate with mercapturic acid, 8-hydroxydeoxyguanosine and 8-nitroguanine. Results The levels of urinary 8-iso-PGF2α in stroke cases were higher than in controls [median (interquartile range), 1.13 (2.23-4.36) μg/g creatinine versus 0.71 (1.34-3.02) μg/g creatinine, p = 0.004]. After adjusting cardiovascular risk factors, the association remained that higher level of urinary 8-iso-PGF2α entailed the greater risk for incident stroke [per 1 standard deviation increase in log-transformed value, adjusted odds ratio, 1.40; 95% confidence interval (CI), 1.06-1.85; p = 0.005] with a significant increasing trend across its quartiles (p for trend = 0.016). After adding urinary 8-iso-PGF2α, the prediction model not only improved discrimination between participants with or without incident stroke (integrated discrimination improvement, 0.025; 95% CI, 0.006-0.045; p = 0.005), but enhanced stroke risk stratification (net reclassification improvement, 19.8%; 95% CI, 4.6-35.1%; p = 0.011). In contrast, the relationships were non-significant among the other three biomarkers. Conclusions Our findings demonstrated that urinary 8-iso-PGF2α could be an independent biomarker of oxidative stress for prediction of the risk for incident stroke. ? 2015 Elsevier Ireland Ltd. All rights reserved.
Subjects
8-Iso-prostaglandin F2α; Nested case-control study; Oxidative stress; Stroke; Urinary biomarkers
Other Subjects
4 hydroxynonenal; 8 hydroxydeoxyguanosine; 8 isoprostaglandin F2 alpha; 8 nitroguanine; acetylcysteine; creatinine; 4-hydroxy-2-nonenal; 8-epi-prostaglandin F2alpha; 8-nitroguanine; 8-oxo-7-hydrodeoxyguanosine; aldehyde; biological marker; creatinine; deoxyguanosine; guanine; prostaglandin F2 alpha; adult; aged; amino acid urine level; Article; cardiovascular risk; case control study; cerebrovascular accident; cohort analysis; controlled study; creatinine urine level; female; human; major clinical study; male; nitrosative stress; oxidative stress; prediction; priority journal; prostaglandin urine level; risk assessment; analogs and derivatives; blood pressure; body mass; cardiovascular disease; cerebrovascular accident; chromatography; middle aged; oxidative stress; physiology; predictive value; procedures; prospective study; risk factor; tandem mass spectrometry; urine; Aged; Aldehydes; Biomarkers; Blood Pressure; Body Mass Index; Cardiovascular Diseases; Case-Control Studies; Chromatography; Creatinine; Deoxyguanosine; Dinoprost; Female; Guanine; Humans; Male; Middle Aged; Oxidative Stress; Predictive Value of Tests; Prospective Studies; Risk Factors; Stroke; Tandem Mass Spectrometry
Publisher
Elsevier Ireland Ltd
Type
journal article