Tumor-infiltrating lymphocytes contain a higher proportion of FOXP3+ T lymphocytes in cervical cancer
Journal
Journal of the Formosan Medical Association
Journal Volume
110
Journal Issue
9
Pages
580-586
Date Issued
2011
Author(s)
Abstract
Background/Purpose: The subpopulations and functions of tumor-infiltrating lymphocytes (TILs) from cervical cancer (CC) are altered. Dysfunction of TIL could be partially because of the inhibition by regulatory T (T reg) cells. FOXP3 is the control gene for the T reg cells. Methods: We investigated the distribution of TILs and FOXP3 + cells in CC (n = 10) and cervical intraepithelial neoplasia (n = 8) tissues. Double-immunofluorescence and confocal-based image quantitative microscopic analysis were used to calculate the number of cluster of differentiation (CD)4 +CD25 +FOXP3 + T reg cells around the tumor cells. Results: The CD4 +CD25 +FOXP3 + phenotype of T reg cells was accumulated around the tumor cells. CC contains a significantly higher proportion of the FOXP3 + T cells than in cervical intraepithelial neoplasia (p < 0.001). Moreover, CC with lymph node metastasis has a higher proportion of the FOXP3 + T cells than that without lymph node metastasis (p < 0.05). Conclusion: The increased accumulation of T reg cells suggests that T reg cells are important in the immunopathogenesis of CC. ? 2011, Elsevier Taiwan LLC & Formosan Medical Association.
SDGs
Other Subjects
transcription factor FOXP3; article; cancer tissue; CD4+ CD25+ T lymphocyte; clinical article; confocal microscopy; female; human; human tissue; immunofluorescence microscopy; immunopathogenesis; lymph node metastasis; phenotype; quantitative analysis; T lymphocyte; tumor associated leukocyte; uterine cervix cancer; uterine cervix carcinoma in situ; Female; Fluorescent Antibody Technique; Forkhead Transcription Factors; Humans; Lymphatic Metastasis; Lymphocytes, Tumor-Infiltrating; Microscopy, Confocal; T-Lymphocytes, Regulatory; Uterine Cervical Neoplasms
Type
journal article