Effect of CDA-II on cell viability, lipid peroxidation, glutathione concentration and its related enzyme activities in primary rat hepatocytes
Resource
The American Journal of Chinese Medicine, 31(3), 415-423
Journal
The American Journal of Chinese Medicine
Journal Volume
31
Journal Issue
3
Pages
415-423
Date Issued
2003
Date
2003
Author(s)
Abstract
This study investigated the effects of various concentrations and incubation time intervals of CDA-II (cell differentiation agent: a preparation of human urine) on cell viability, lipid peroxidation and glutathione and its related enzyme activities in rat hepatocytes. Based on the results of lactate dehydrogenase leakage and microscopic examination, treatment with CDA-II significantly elevated the viability of hepatocytes. The level of lipid peroxidation of cells treated with 2.5 or 5 mg/ml CDA-II was lower than the control after 4, 8 and 24 hours of incubation. Intracellular glutathione (GSH) content of cells treated with 0.25-5 mg/ml CDA-II was significantly higher than controls after 8 and 24 hours of incubation. These phenomena are beneficial to the antioxidant capabilities of hepatocytes. Furthermore, treatment of hepatocytes with CDA-II has no effect on the activities of GSH peroxidase, GSH reductase and GSH S-transferase. In conclusion, adequate concentration of CDA-II could enhance the viability and antioxidative capability of hepatocytes. ? 2003 World Scientific Publishing Company & Institute for Advanced Research in Asian Science and Medicine.
Subjects
CDA-II; Glutathione; Hepatocytes; Human Urine; Rat
SDGs
Other Subjects
cell differentiation agent II; glutathione; glutathione peroxidase; glutathione reductase; glutathione transferase; lactate dehydrogenase; peptide; phenylacetic acid; unclassified drug; animal cell; antioxidant activity; article; cell differentiation; cell viability; controlled study; drug effect; enzyme activity; incubation time; lipid peroxidation; liver cell; nonhuman; rat; Animals; Cell Differentiation; Cell Survival; Cells, Cultured; Dose-Response Relationship, Drug; Free Radical Scavengers; Glutathione; Hepatocytes; Humans; Lipid Peroxidation; Liver; Male; Peptides; Phenylacetates; Rats; Rats, Sprague-Dawley; Time Factors; Urine
Type
journal article
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