Time Domain Analysis of Protein Complexes Using An IGZO Thin Film Transistor-based Biosensor Integrated with Microfluidic Channels

In this thesis, bio-chemical reaction and protein complexes interaction are detected by an IGZO thin film transistor-based (TFT-based) biosensor. These two issues are discussed separately in two parts. In the first part, a bio-chemical reaction is detected. This reaction is a part of the metabolic pathway and it is a step in malate-aspartate shuttle, which is one of the most important bio-chemical system in human body. The reaction system involving different concentrations of composition are investigated. By measuring the electrical signals of TFT, the relation between current change and complete/incomplete reactions is revealed. The second part is about the detection of Streptavidin-Biotin protein complexes. The TFT-based biosensor is integrated with microfluidic channels in order to investigate the diffusion behaviors of target analytes. The diffusion experiments of Streptavidin and Biotin were first conducted separately to understand the diffusion properties. Then the binding interaction of Streptavidin-Biotin complexes is detected and analyzed in time domain. We also verified the binding among Streptavidin and Biotin by using the fluorescence microscope.