Accurate quantitation of glutathione in cell lysates through surface-assisted laser desorption/ionization mass spectrometry using gold nanoparticles
Journal
Nanomedicine Nanotechnology Biology and Medicine
Journal Volume
6
Journal Issue
4
Pages
530-537
Date Issued
2010
Date
2010
Author(s)
Abstract
We developed a method for the determination of three aminothiols-cysteine, glutathione (GSH), and homocysteine-using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). The analytes were first captured using the unmodified 14-nm gold nanoparticles; N-2-mercaptopropionylglycine-modified gold nanoparticles serving as internal standard were sequentially added, and then the sample was analyzed using SALDI-MS. This approach provided good quantitative linearity of the three analytes (R2 = ~0.99), with good reproducibility (relative standard deviations: <10%), in the analyses of GSH in the lysates of human red blood cells and MCF-7 cancer breast cells in the presence and absence of the anti-inflammatory drug sulfasalazine. The internal-standard SALDI-MS approach provides simplicity, accuracy, and precision to the determination of GSH in cells under drug invasion, to open an avenue for SALDI-MS to be used for the precise quantitative determination of a variety of analytes. From the Clinical Editor: This paper reports the development of a surface assisted laser desorption/ionization mass spectrometry method to precisely determine aminothiols-cysteine (Cys), glutathione (GSH), and homocysteine (HCys). ? 2010 Elsevier Inc.
Subjects
Aminothiols; Drug therapy; Gold nanoparticles; Internal standard; Mass spectrometry
SDGs
Other Subjects
Aminothiols; Analytes; Anti-inflammatory drugs; Glutathiones; Gold Nanoparticles; Homocysteines; Human red blood cell; In-cell; Internal standards; Lysates; Quantitative determinations; Relative standard deviations; Reproducibilities; Surface-assisted laser desorption/ionization; Blood; Desorption; Gold; Mass spectrometry; Nanoparticles; Standards; Drug therapy; cysteine; glutathione; gold nanoparticle; homocysteine; salazosulfapyridine; tiopronin; accuracy; article; cell lysate; cell strain MCF 7; controlled study; erythrocyte; human; human cell; mass spectrometry; particle size; quantitative analysis; reproducibility; standard; surface assisted laser desorption ionization mass spectrometry; Erythrocytes; Glutathione; Gold; Homocysteine; Humans; Metal Nanoparticles; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tumor Cells, Cultured
Type
journal article
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