The Effects and Mechanism of Resveratrol on Proliferation of K562 Cells
Date Issued
2004
Date
2004
Author(s)
Cheng, Pei-Yu
DOI
en-US
Abstract
Resveratrol (3, 5, 4’-trihydroxystilbene) is a multifunctional natural product that is abundant in many plants. Previous studies have demonstrated that resveratrol is capable of inhibiting the initiation, promotion and progression of carcinogenesis; interfering with cell-signaling transduction, inducing cell apoptosis; and promoting cell differentiation. In this study, low dosage of resveratrol was found to induce erythroid differentiation in K562 cells. On the other hand, inhibition of the proliferation of K562 cells was found in highly dosage and that was likely due to the inhibition of cellular DNA synthesis. Further study showed that high dosage of resveratrol caused cell cycle arrested at G2/M phase and induced cell apoptosis, which was shown by the increases in sub-G1 cell population and TUNEL assay. The arrest of cells in G2/M phase may be caused by the disturbance of cell division. Subsequently, the study was focused on topoisomerases since they are among the important enzymes involved in the above mechanisms. In vitro relaxation and decatenation assay showed that resveratrol specifically inhibited the activity of topoisomerase II which could account for the DNA synthesis inhibition. The results obtained from electrophoretic mobility shift assay and surface plasmon resonance study indicated that the resveratrol affects the interaction of DNA duplex and topoisomerase II. This is the first report to demonstrate the inhibition of topoisomerase II by resveratrol.
Subjects
拓樸異構酶
白藜蘆醇
K562 cell line
topoisomerase
resveratrol
Type
other
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