Reduction of Phytic Acid in Soymilk by Immobilized Phytase System
Journal
Journal of Food Science
Journal Volume
83
Journal Issue
12
Pages
2963-2969
Date Issued
2018
Author(s)
Abstract
In this study, three carriers (glass microsphere, cellulose beads and AlgNa/PVA beads) were evaluated as phytase solid carriers for reduction of phytic acid within soymilk. Phytase was covalently immobilized onto or entrapped within carriers for repeated use. Glass microsphere was chosen due to its high catalytic efficiency. Optimal operating condition (pH 6.0, 60 ¢XC) was determined using 4-Nitrophenyl phosphate disodium salt hexahydrate as an indicator. Operational reusability was confirmed for more than seven batch reactions and the storage stability was capable of sustaining 70% of its catalytic activity for 40 days. The kinetic parameters including rate constant (K), time (£n 50 ) in which 50% of phytic acid hydrolysis was reached, and time (£n complete ) required to achieve complete phytic acid hydrolysis, were 0.023 min ?1 , 35.7 min, 110 min. The current procedure provides a cheap as well as an easy way to carry out the reduction of phytic acid in soymilk, which has great potential in practical application. ? 2018 Institute of Food Technologists?
Subjects
enzyme immobilization system
glass microsphere
phytase
phytic acid
soymilk
SDGs
Type
journal article