行政院國家科學委員會專題研究計畫 期中精簡報告:細胞凋亡調控基因(sFRP2)在犬乳腺腫瘤之細胞凋亡調控角色及相關調節因子的研究(1/3)
Date Issued
2003
Date
2003
Author(s)
DOI
912313B002404
Abstract
Mammary neoplasms are important and common tumors in both animals and humans and the etiology is complex. The secreted frizzled related proteins (sFRPs) are newly identified proteins and implicated to have dual roles of modulation of Wnt-Frizzled signal transduction pathway and regulation of apoptosis. We have recently found that sFRP2 was expressed abundantly in human and canine mammary gland tumors (MGT) tissues but was undetectable in normal canine mammary gland. To systematically investigate the functional roles and molecular mechanisms of sFRP2 in canine MGT, several strategies are to be carried out as described below.
The project is comprised of six major parts for a period of 3 years: In the 1st year, new primary cell cultures from native canine MGT tissues has been established and purified for mammary epithelial cells. We have successfully established and analyzed more native primary MGT cell lines from surgically excised MGT specimens. The cells are characterized for their cell origins, proliferation rate (by MTT assay), expression of sFRP2 by RT-PCR, in situ hybridization, and immunohistochemistry, and Western blotting. Expression experiments revealed the sFRP2 was abundantly expressed in canine MGT cell lines, but not expressed in normal canine MG cells nor other commercial non-MGT cell lines. Canine sFRP2 is cloned into a mammalian expression vector with GFP reporter gene and CMV promoter. The GFP-sFRP2 is stably transfected into primary canine MGT and commercial MGT cell lines by lipofection for further analysis feom the next stage of the project.
The results of this stage of the project should offer important scientific basis and information to understand the roles of sFRP gene family in canine neoplastic cells. It also provides a basis for further analysis of functions of different members of the sFRP gene family and elucidation of the complex etiology of canine model of mammary tumors.
Subjects
secreted apoptosis related protein secreted frizzled related protein
apoptosis
gene expression
gene transfection
mammary neoplasia
Publisher
臺北市：國立臺灣大學獸醫學系暨研究所
Type
report
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