Analysis of amino acids and biogenic amines in breast cancer cells by capillary electrophoresis using polymer solutions containing sodium dodecyl sulfate
Journal
Journal of Chromatography A
Journal Volume
1217
Journal Issue
4
Pages
582-587
Date Issued
2010
Date
2010
Author(s)
Abstract
We describe simultaneous analysis of naphthalene-2,3-dicarboxaldehyde (NDA)-amino acid and NDA-biogenic amine derivatives by CE in conjunction with light-emitting diode-induced fluorescence detection using poly(ethylene oxide) (PEO) solutions containing sodium dodecyl sulfate (SDS). After sample injection, via EOF 0.1% PEO prepared in 100 mM TB solution (pH 9.0) containing 30 mM SDS entered a capillary filled with 0.5 M TB solution (pH 10.2) containing 40 mM SDS. Under this condition, 14 NDA-amino acid and NDA-amine derivatives were separated within 16 min, with high efficiency ((1.0-3.2) × 105 theoretical plates) and sensitivity (LODs at S/N = 3 ranging from 2.06 to 19.17 nM). In the presence of SDS and PEO, analytes adsorption on the capillary wall was suppressed, leading to high efficiency and reproducibility. The intraday analysis RSD values (n = 3) of the mobilities for the analytes are less than 0.52%. We have validated the practicality of this approach by quantitative determination of 9 amino acids in breast cancer cells (MCF-7) and 10 amino acids in normal epithelial cells (H184B5F5/M10). The concentrations of Tau and Gln in the MCF-7 cells were different than those in the H184B5F5/M10 cells, respectively. Our results show the potential of this approach for cancer study. ? 2009 Elsevier B.V. All rights reserved.
Subjects
Amino acids; Biogenic amines; Human breast cancer cell; Light-emitting diode-induced fluorescence detection; Micellar electrokinetic chromatography; Poly(ethylene oxide); Sodium dodecyl sulfate
SDGs
Other Subjects
Biogenic amines; Fluorescence detection; Human breast cancer cells; Micellar electrokinetic chromatography; Sodium dodecyl sulfate; Adsorption; Amination; Amino acids; Capillary electrophoresis; Cells; Chromatographic analysis; Chromatography; Current density; Diffusers (optical); Diodes; Electrodynamics; Ethylene; Financial data processing; Fluorescence; Insecticides; Light emission; Light emitting diodes; Naphthalene; Organic acids; Polyethylene oxides; Polymer solutions; Polymorphism; Sodium; Sodium sulfate; Terbium alloys; Amines; 2,3 naphthalenedicarboxaldehyde; alanine; amino acid; aspartic acid; biogenic amine; dodecyl sulfate sodium; glutamic acid; glutamine; glycine; histidine; macrogol; serine; taurine; tyrosine; valine; adsorption; amino acid analysis; article; breast cancer; cancer cell; capillary electrophoresis; controlled study; epithelium cell; fluorescence analysis; human; human cell; light emitting diode; micellar electrokinetic chromatography; priority journal; quantitative analysis; reproducibility; sensitivity analysis; validation study; Amino Acids; Biogenic Amines; Breast Neoplasms; Cell Line, Tumor; Electrophoresis, Capillary; Female; Fluorescence; Humans; Limit of Detection; Naphthalenes; Polyethylene Glycols; Reproducibility of Results; Sodium Dodecyl Sulfate
Type
journal article
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