Proteomic changes associated with metabolic syndrome in a fructose-fed rat model
Journal
Journal of Food and Drug Analysis
Journal Volume
24
Journal Issue
4
Pages
754-761
Date Issued
2016
Author(s)
Abstract
Metabolic syndrome (MetS), characterized by a constellation of disorders such as hyperglycemia, insulin resistance, and hypertension, is becoming a major global public health problem. Fructose consumption has increased dramatically over the past several decades and with it the incidence of MetS. However, its molecular mechanisms remain to be explored. In this study, we used male Sprague-Dawley (SD) rats to study the pathological mechanism of fructose induced MetS. The SD rats were fed a 60% high-fructose diet for 16?weeks to induce MetS. The induction of MetS was confirmed by blood biochemistry examination. Proteomics were used to investigate the differential hepatic protein expression patterns between the normal group and the MetS group. Proteomic results revealed that fructose-induced MetS induced changes in glucose and fatty acid metabolic pathways. In addition, oxidative stress and endoplasmic reticulum stress-related proteins were modulated by high-fructose feeding. In summary, our results identify many new targets for future investigation. Further characterization of these proteins and their involvement in the link between insulin resistance and metabolic dyslipidemia may bring new insights into MetS. ? 2016
Subjects
endoplasmic reticulum stress; fructose; insulin resistance; metabolic syndrome; oxidative stress
SDGs
Other Subjects
cholesterol; dihydrolipoamide acetyltransferase; dihydroxyacetone phosphate acyltransferase; fatty acid; fatty acid synthase; fructose; fructose 1,6 biphosphatase 1; glial fibrillary acidic protein; glucose; glucose regulated protein 78; glutathione s transferase alpha 3; insulin; liver protein; long chain fatty acid coenzyme A ligase; peroxiredoxin 1; Rho guanine nucleotide dissociation inhibitor 1; thiobarbituric acid reactive substance; triacylglycerol; unclassified drug; fructose; insulin; animal experiment; animal model; animal tissue; Article; blood biochemistry; carbohydrate diet; controlled study; endoplasmic reticulum stress; fatty acid metabolism; glucose metabolism; male; metabolic syndrome X; metabolism; nonhuman; oxidative stress; pathogenesis; protein expression; proteomics; rat; animal; disease model; glucose blood level; metabolic syndrome X; proteomics; Sprague Dawley rat; Animals; Blood Glucose; Disease Models, Animal; Fructose; Insulin; Male; Metabolic Syndrome; Proteomics; Rats; Rats, Sprague-Dawley
Type
journal article