Research and Development on Using a Quartz Crystal Microbalance Platform to Integrate Surface Plasmon Resonance and Electrochemical Impedance Spectroscopy
Date Issued
2010
Date
2010
Author(s)
Lin, Yu-Chieh
Abstract
Bio-affinity measurement is always an important issue in bio-technology research field. Since the interaction process between bio-molecules is complex, more parameters are needed for facilitating the analysis. In this study, Quartz Crystal Microbalance (QCM) was provided as a base platform. Surface Plasmon Resonance (SPR) and Electrochemical Impedance Spectroscopy (EIS) functions were added on QCM to form a novel integrated system. That is, the change in resonance frequency and dissipation factor from QCM, the optical power change from SPR and the Impedance change from EIS could be simultaneously measured.
In this system, the bottom electrode of QCM was replaced with ITO and the prism coupling was used to excite SPR. The upper electrode of QCM was used as the working electrode for EIS function and a switching circuit was used for measurement of this two system by turns. The infrastructure of this integrated system not only provide multi-parameter measurement but also keep their own features.
According to our experimental results, we found that the use of coupling oil changed the features of QCM. That means there exists some problem when integrating these three system with prism coupling excitation. But the integration of QCM and EIS, SPR and EIS is successful.
Subjects
QCM
SPR
EIS
File(s)![Thumbnail Image]()
Loading...
Name
ntu-99-R97525026-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):93a46c32325d237a63774d84a51e4750