Conditionally interfere the migration of zebrafish (Danio rerio) Primordial Germ Cells
Date Issued
2005
Date
2005
Author(s)
Hsieh, Tsung-Che
DOI
zh-TW
Abstract
In embryogenesis of zebrafish, primordial germ cells (PGCs) formed four clumps before 1000-cell stage. PGCs would migrate and form two bilateral rows of cells close to the future gonad site. We micro-injected with a BstBI-cut pZBrBISRK plasmid and generated 5 transgenic line. One of which, ZBrBISRK2, having 45 % heredity rate, the highest among 5 transgenic lines, was selected. The nanos mRNA was detected not only in future gonad but also in head, trunk, and tail in the F2 embryos at 30 hpf derived from transgenic line ZBrBISRK2, whereas nanos mRNA was only detected in the gonad of wild type embryos, suggesting there were the mis-migrated PGCs. In Western blot, we could detect the positive bands in the total proteins extracted from ZBrBISRK2 F2 embryos. Therefore, we conclude that using conditionally regulatory system results in interfering the PGCs migration.
Subjects
斑馬魚
zebrafish
Type
other
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