The Differentially Expressed Genes in Pre-lay and Laying Geese
Date Issued
2009
Date
2009
Author(s)
Yen, Cheng-Feng
Abstract
Geese have a short egg laying period and a low egg production rate. In order to induce and maintain egg laying, genes related to generating hepatic lipid for yolk deposition should be adequately expressed. Liver mRNA from 6 geese was extracted and used for construction of full length enriched cDNA library. About 2400 gene sequences were determined and NCBI/GGGI data bases were used to analyze these sequences, 10 highly expressed genes were selected for further research. The tissue distribution data showed that ApoVLDL II, liver type fatty acid binding protein (L-FABP), vitellogenin I and vitellogenin II transcripts were specifically expressed in the liver of laying geese. Ovoinhibitor, preproalbumin, -2-hs-glycoprotein and vitamin D binding protein mRNA were highly expressed in the liver and to a lesser extend in other tissues. Ovotransferrin mRNA was expressed in the liver, ovary, oviduct, shell gland, brain and adipose tissue. The concentrations of hepatic ovotransferrin, ovoinhibitor, preproalbumin, ApoVLDL II, vitellogenin I, vitellogenin II and vitamin D binding protein mRNA were higher in the liver of laying geese than in pre-lay geese, suggesting that these genes may involved in laying function or lipid metabolism related to egg formation.We also used a suppression subtractive hybridization technique to explore the genes which were specifically expressed in the liver and pituitary gland of laying geese. We found that there were 13 genes, including apoVLDL II, vitellgenin I, ethanolamine kinase, G-protein gamma-5 subunit, leuayl –tRNA synthase, and 8 novel genes specifically expressed in the liver of laying geese. Several of these differentially expressed genes were found to be responsive to estrogen stimulation, confirming the involvement of these genes in egg laying function of the goose. We also found that there were 6 genes, including prolactin, visinin-like protein and 4 novel genes highly expressed in the pituitary gland of laying geese. he effect of dietary DHA and selenium (Se) supplementation on the mRNA expression of lipogenic genes in livers of lying geese were also examined. Laying geese were fed with 3.5% fish oil diets containig different Se concentration (0, 0.15, 0.3 mg/kg) for 3 month. The mRNA concentration of sterol regulatory element binding proteins 1, malic enzyme, stearoyl CoA desturase 1 and fatty acid synthease were determined. Dietary DHA and Se supplement had no effect on the lipogenic genes expression in the liver of laying geese. aken together, the data show that there are laying specific genes that may involve in supporting the laying physiology for the geese, and hepatic lipogenic genes of laying geese were not affected by DHA and Se treatment.
Subjects
cDNA library
Goose
Lipogenesis
Type
thesis
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