Imaging granularity of leukocytes with third harmonic generation microscopy
Journal
Biomedical optics express
Journal Volume
3
Journal Issue
9
Date Issued
2012-09-01
Author(s)
Tsai, Cheng-Kun
Chen, Yu-Shing
Wu, Pei-Chun
Hsieh, Tsung-Yuan
Liu, Han-Wen
Yeh, Chiou-Yueh
Liu, Tzu-Ming
Abstract
Using third harmonic generation (THG) microscopy, we demonstrate that granularity differences of leukocytes can be revealed without a label. Excited by a 1230 nm femtosecond laser, THG signals were generated at a significantly higher level in neutrophils than other mononuclear cells, whereas signals in agranular lymphocytes were one order of magnitude smaller. Interestingly, the characteristic THG features can also be observed in vivo to track the newly recruited leukocytes following lipopolysaccharide (LPS) challenge. These results suggest that label-free THG imaging may provide timely tracking of leukocyte movement without disturbing the normal cellular or physiological status.
Subjects
(170.1530) Cell analysis; (180.4315) Nonlinear microscopy
Type
journal article