Cancer Cell Identification by Bi-color ZnO and TiO2 Nanowires
Date Issued
2014
Date
2014
Author(s)
Li, Wei-Jen
Abstract
Among cancer cell detection methods, fluorescence microscopy cancer detection is one of the most common methods. However, there are many disadvantages for fluorescence cancer detection such as the phototoxicity, the limited number of available fluorescent channels, and the overlap of the excitation and emission spectra of the stains. Furthermore, under a constant light illumination, it possesses the issue of photobleaching, making real-time surgery difficult. Many groups have reported that quantum dots can conquer these problems; however, the quantum dots method requires surface modification and the surface state induced by modification changes the property of semiconductor material. Moreover, all the processes cost high.
To overcome these issues, the semiconductor light sources are alternative choices. ZnO and TiO2 nanowires connected with specific antibodies are proposed to identify cancer cells and normal cells, replacing the organic fluorescent substances in the traditional cancer detection method. ZnO and TiO2 have exceptional optical properties, and they are often applied to biomedical research and commercial products. In addition, duto to great affinities between both ZnO and TiO2 nanowires and many proteins, ZnO and TiO2 nanowires bounded to specific antibodies are regarded as biomarkers to distinguish cancer cells from normal cells. Furthermore, from PL spectra and bio-images, our idea for cancer cell detection by semiconductor nanowires was confirmed.
Then, a series of quantitative analyses, including biomarker concentration limit, the valid range of cell numbers, and co-culture case to simulation the real situation were conducted to examine the relationship between the optical response from biomarkers and cell numbers. The data from quantitative analyses were expected to provide a reference for surgeon for real-time cancer cell detection while performing the surgery.
In this thesis, we reported a novel cancer cell detection technology by using ZnO and TiO2 nanowires connected to specific antibodies to distinguish cancer cells from normal cells, leading successful real-time cancer cell detection during cancer resection. Moreover, a series of quantitative experiments will provide physicians with key parameters and thus systemized the real-time cancer cell detection.
Subjects
癌症細胞檢測
水熱法
氧化鋅
二氧化鈦
奈米線
生物量化
SDGs
Type
thesis
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