Numerical Study of Spectrally-resolved Third Harmonic Generation Microscopy
Date Issued
2015
Date
2015
Author(s)
Lee, Chien-Ming
Abstract
By computing the response function of the electric field and the nonlinear susceptibility, I build up a simulation model for the numerical study of third harmonic generation microscopy based on the non-instantaneous third harmonic generation process for the case the response times are comparable. Based on the IR spectrum of a broadened Cr:forsterite laser and the absorption spectrum of a possible contrast agent, linoleic acid, we found six parameters including field pulse-width, envelope form, second-/third-order dispersions, de-phasing times, resonant to non-resonant ratios, and resonant wavelengths that could affect the response function, thus would change the resulting third harmonic generation (3HG) in both intensity and shape spectrally. Since we are aimed at identifying the molecular resonance in 3HG spectrum, the ratio of the intensity at one-third of medium resonant peak over the intensity at one-third of laser peak were calculated. By finding the range the intensity ratio is larger than 1, we could obtain the acceptable range of the parameters for resolving the spectral characteristics of resonant components which makes possible the spectrally-resolved 3HG microscopy.
Subjects
Non-instantaneous Polarization
Third Harmonic Generation Microscopy
Numerical Study
Temporal Response Function
Spectrally-resolved
Resonant Susceptibility
Type
thesis
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