Development of a novel biochip for rapid multiplex detection of seven mastitis-causing pathogens in bovine milk samples.
Resource
J Vet Diagn Invest,20(4),463-471.
Journal
J Vet Diagn Invest
Journal Volume
20
Journal Issue
4
Pages
463-471
Date Issued
2008-10
Date
2008-10
Author(s)
Lee K. H.
Lee, J. W.
Wang, S.W.
Liu, L. Y.
Lee, M. F.
Chuang, S. T.
Shy, Y. M.
Chang, C. L.
Wu, M. C.
Abstract
To efficiently prevent and treat bovine mastitis and minimize its effect on the dairy industry, a sensitive, rapid, and specific test is required for identifying the mastitis-causing pathogens. In this study, a biochip capable of detecting 7 common species of mastitis-causing pathogens, including Corynebacterium bovis, Mycoplasma bovis, Staphylococcus aureus, and the Streptococcus spp. S. agalactiae, S. bovis, S. dysgalactiae, and S. uberis, within 6 hr was developed. The technique is based on DNA amplification of genes specific to the target pathogens and consists of 4 basic steps: DNA extraction of bacteria, polymerase chain reaction, DNA hybridization, and colorimetric reaction. To examine the accuracy and specificity of this biochip, a preliminary test with 82 random quarter milk samples were analyzed and compared with results from conventional microbiological methods conducted simultaneously. Results from all but 1 sample analyzed by the biochip were in agreement with those analyzed by bacteriology. The biochip could be a feasible tool for rapidly diagnosing mastitis-causing pathogens in milk and providing information for a more effective treatment to cure mastitis.