Inhibitory effect of resveratrol on angiotensin II-induced cardiomyocyte hypertrophy.
Resource
Naunyn Schmiedebergs Arch Pharmacol., 369(2):239-44
Journal
Naunyn-Schmiedeberg s Archives of Pharmacology
Pages
239-244
Date Issued
2004-02
Date
2004-02
Author(s)
Cheng TH
Liu JC
Lin H
Shih NL
Chen YL
Huang MT
Chan P
Cheng CF
Chen JJ.
Abstract
Resveratrol is proposed to account in part for the protective effect of red wine on the cardiovascular system. Angiotensin II (Ang II) is a potent hypertrophic stimulus in cardiomyocytes. In this study, we determined the effect of resveratrol on Ang II-induced cardiomyocyte hypertrophy. Cultured neonatal rat cardiomyocytes were stimulated with Ang II, and [3H]leucine incorporation and beta-myosin heavy chain (beta-MyHC) promoter activity were examined. Intracellular reactive oxygen species (ROS) were measured by a redox-sensitive fluorescent dye, 2' 7'-dichlorofluorescin diacetate, and the extracellular signal-regulated kinase (ERK) phosphorylation was examined by Western blotting. Resveratrol inhibited Ang II-increased intracellular ROS levels. Furthermore, resveratrol, as well as the antioxidant N-acetyl-cysteine, decreased Ang II- or H2O2-increased protein synthesis, beta-MyHC promoter activity, and ERK phosphorylation. In summary, we demonstrate for the first time that resveratrol inhibits Ang II-induced cardiomyocyte hypertrophy via attenuation of ROS generation.
SDGs
Type
journal article