Screening of lectins by an enzyme-linked adsorbent assay
Journal
Food Chemistry
Journal Volume
113
Journal Volume
113
Journal Issue
4
Journal Issue
4
Pages
1218-1225
Start Page
1218
End Page
1225
ISSN
03088146
Date Issued
2009-04-15
Author(s)
Abstract
This study provides a new strategy to detect the specific carbohydrate binding capability of lectins. A sugar-polymer based enzyme-linked adsorbent assay was established by applying different monosaccharide-polyacrylamide conjugates as capturing agents for screening lectins in biological samples. Four model lectins, concanavalin A (Con A), wheat germ agglutinin (WGA), soybean agglutinin (SBA) and Ulex europaeus agglutinin I (UEA I), were employed as the comparative lectins against each corresponding sugar. The results suggested that this assay is more sensitive than the conventional hemagglutinating methods. On the screening of specific carbohydrate binding capability from biological materials, extracts of papaya (Carica papaya) seeds, longan (Euphoria longana) seeds and sorghum (Sorghum bicolor) were shown various specific monosaccharide binding capabilities which had not been reported before. In conclusion, the assay provides an alternative and efficacious detection for the specific carbohydrate binding substances in biological samples.
Subjects
ELISA
Hemagglutination
Lectin
Monosaccharide
Screening
Type
journal article