Fabrication of Gelatin-Based Adhesives with Various Crosslinking Mechanisms
Date Issued
2016
Date
2016
Author(s)
Liu, Yi
Abstract
Tissue adhesives have been developed to overcome the limitations of conventional wound closure (e.g. sutures and staples) including further damage and no stop body fluid and gas. Tissue adhesives offer some advantages such as simple implementation procedure, less painful, and no need for removal. However, cyanoacrylates and fibrin glues, the representative types of adhesives, are limited by toxicity and low adhesion, respectively. In this study, gelatin was chosen as backbone of adhesive, due to the biocompatibility, biodegradability, and low cost. For increasing the mechanical properties and tissue adhesion of the gelatin-based adhesives, modified gelatin and double-crosslinking mechanism were applied in photo-crosslinked gelatin and Fe(III)-crosslinked dopamine-modified gelatin, respectively. Firstly, the dopamine- modified gelatin and phloretic acid-modified gelatin were synthesized. Then, gelatin-based adhesives were formed including photo-crosslinked gelatin (PG), photo-crosslinked phloretic acid-modified gelatin (PPG), photo-crosslinked dopamine-modified gelatin (PDG), photo-Fe(III)-crosslinked dopamine-modified gelatin (PFDG), Fe(III)-crosslinked dopamine-modified gelatin (FDG), and Fe(III)-H2O2- crosslinked dopamine-modified gelatin (FHDG). The swelling properties, degradation properties, rheology, tissue adhesion, and cytotoxicity of gelatin-based adhesives were measured. In the results, gelatin-based adhesives showed the low swelling index, great biodegradability, and great biocompatibility (except FHDG). In results of single photo-crosslinked adhesives, PPG showed the greatest viscoelasticity and highest tissue adhesive strength, because the photo-crosslinking density increased with addition of phenol group. In results of improving Fe(III)-crosslinked adhesives, FHDG showed the highest viscosity due to the application of both ion-crosslinking (Fe(III)-catechol) and oxidation-crosslinking (catechol-catechol), but the cytotoxicity limited it. The research presented potential of gelatin-based adhesives for wound closure and healing.
Subjects
Tissue adhesive
Modified gelatin
Dopamine
Phloretic acid
Fe(III)-crosslinking
Photo-crosslinking
Type
thesis